Methods Cirrhosis was induced in BALB/c mice by means of injection with 50% carbon tetrachloride for 8W. Hepatocytes were isolated from cirrhotic and normal mice by means of an in situ perfusion method with collagenase solution with minor modifications.
方法 通过50％CCL4腹腔注射，用8W时间诱发BALB／c小鼠形成肝硬化模型。 应用改良的胶原酶原位灌注法分离正常和肝硬化小鼠的肝细胞。
参考来源 - TGF—β1对肝细胞再生分化及凋亡的调控作用机制Methods The drug concentration by in situ perfusion in rats was determined by HPLC and the volume of perfusion fluid was adjusted by Phenol red labeling.
方法应用大鼠在体胃肠吸收模型,采用酚红标记法校正肠循环液的体积,HPLC法测定不同时间点胃肠循环液中荆芥内酯的质量浓度。
参考来源 - 荆芥内酯大鼠在体胃肠吸收动力学研究·2,447,543篇论文数据，部分数据来源于NoteExpress
Methods The absorption kinetics and absorption sites were investigated by using in situ perfusion method in rats.
方法采用大鼠在体肠灌流方法，研究卡马西平大鼠肠吸收动力学。
METHODS: The absorption kinetics and absorption site of oxymatrine were investigated using in situ perfusion method in rats.
方法采用大鼠在体肠灌流吸收实验，研究了氧化苦参碱的吸收部位和吸收动力学特征。
Method the absorption kinetics of harmine hydrochloride from duodenum, jejunum, ileum, and colon were investigated using in situ perfusion method in the rats.
方法采用大鼠在体肠循环法分别研究盐酸去氢骆驼蓬碱在大鼠十二指肠、空肠、回肠及结肠中的吸收动力学特征。
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